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采用STR和SNP遗传标记鉴定全同胞姐妹关系

法医学杂志, Journal of Forensic Medicine,2010年03期
采用STR和SNP遗传标记鉴定全同胞姐妹关系


【作者】 张素华; 赵书民; 李莉;
【Author】 ZHANG Su-hua1,2,ZHAO Shu-min2,LI Li2(1.Department of Forensic Medicine,Medical College of Soochow University,Suzhou 215123,China;2.Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,P.R.China,Shanghai 200063,China)
【机构】 苏州大学医学部法医学系; 司法部司法鉴定科学技术研究所上海市法医学重点实验室;
【摘要】 目的通过对常染色体和X染色体遗传标记的检测,探讨全同胞姐妹关系的鉴定策略。方法提取姐妹个体的DNA,采用SinofilerTM试剂盒检验常染色体上的15个STR基因座、采用Mentype Argus X-8试剂盒和多重X染色体STR检测试剂盒检验X染色体上的17个STR基因座,同时采用TaqMan技术对11个X-SNP位点进行分型检测。结果依据常染色体STR基因座的检测结果计算全同胞指数,不排除被检个体的同胞姐妹关系;X染色体上各个STR基因座和SNP位点均检见1~2个相同的等位基因,进一步支持被检个体的同胞姐妹关系。结论对于全同胞姐妹关系的鉴定案例,除了检测常染色体STR基因座外,还可以从X染色体上选择多态性遗传标记进行检测,获得更多的遗传信息。 更多还原

【Abstract】 Objective The methods for identification of sibling sisters were explored with detection of genetic markers on autochromosome and X-chromosome.Methods Genomic DNA of the sibling sisters were extracted,and 15 STRs on autochromosome and 17 STRs on X-chromosome were genotyped by SinofilerTM kit,Mentype Argus X-8 kit and in-house kit of X-STRs,respectively.11 X-SNPs were genotyped with TaqMan technology.Results Full sibling relationship of the test samples were confirmed by calculating full sibling index of STRs in autochromosome,which were also supported by the detection of 1-2 same alleles at each locus on X-chromosome.Conclusion In identification of full sibling sister,not only STRs on autochromosome but also polymorphism genetic markers in X-chromosome can be utilized. 更多还原
【关键词】 法医遗传学; 同胞关系; X染色体; 短串联重复序列; 单核苷酸多态性;

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