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渤海國的mtDNA

本帖最后由 Anthro 于 2015-10-29 13:37 编辑

Experience on Sequencing of Mitochondrial DNA from 1200 Year Old Bone Using Cloning

Abstract

Ancient bones have undergone natural decomposition and have been exposed to external environment for long period. Ancient DNA from old bone is usually fragmented. In addition, various kinds of inhibitors are co-extracted. All these may inhibit proper sequencing reaction. Cloning is regarded as the standard method when sequencing aDNA. When cloning, each clone from the same sample may not be of same sequence, and to exact consensus sequence may be difficult. Here we present our experience on 1200 year old bone from Russia, Primorsky Kray area. We have tried to sequence for HV I, II region of mtDNA using modified mini-primer set, which consisted of 7 set to cover the HV I, II. We cloned the PCR product and sequenced all the clones. Amplification efficiency and subsequent success rates were different for each mini primer set. Loci of variation that differ from consensus sequences were rather frequent, and the pattern were variable depending on sample. Except major polymorphic sites that are important when haplogroup designation, 16129 was the most frequent site that was discarded when extracting haplogroup designation.

本遺址Chernyatino-5古墓是位于俄羅斯濱海邊疆區Oktyabrsky區Chernyatino村

Open Access

http://www.kjlm.or.kr/v1/bbs/board.php?bo_table=abstract&wr_id=114&no=1
1

评分次数

年底出了一堆古dna研究,中国这么庞大的研究资源,相对而言,出来的成果,最近一年真是少之又少。
新技术方案尝试:低覆盖全基因组,最低成本深度解析父系源流,略有成效,大家一起摸索。微博@好奇云怪 QQ群:387100816。
9个D4 1个N9a 1个F 1个M9a
年底出了一堆古dna研究,中国这么庞大的研究资源,相对而言,出来的成果,最近一年真是少之又少。
Yungsiyebu 发表于 2015-2-11 22:16
研究资源庞大,但国内对这个重视程度还是比较低。古DNA做的好的也就吉大和复旦。像中科院、哈尔滨医科大、中科院昆明所等单位的资源都是浪费。总体上,中国的政府和社会对这类基础研究的重视不够。
那M9a的下游有测过吗?五六年前的文章了
那M9a的下游有测过吗?五六年前的文章了
重返榮耀 发表于 2015-2-13 02:27
7个D4  1个D4i  1个D4b1 1个F 1个N9a3 1个M9a*
韩文的文献啊,渤海国本身民族成分复杂,滨海地区应该更接近女真成分。
7# neuche
但是靺鞨语言上跟满语好像不近呢
8# 红豆吐司 满语诞生时间不长,清初才从蒙古文借鉴后创立,还没来的及推广,满人皇帝自己都不会说了。
8# 红豆吐司 还有一点,满人应该是相对通古斯人来说更靠近辽东汉人和北朝鲜人的,而这以一遗址是在俄罗斯的滨海地区,属于野人女真的领地,而野人女真并未算在满八旗以内。
10# wanhuatong
那么我可以理解成你也同意靺鞨跟满族其实没什么直系的承袭关系吗?

其实我是怀疑北汉大量的母系来自渤海国,高句丽人,但是他们的父系哪去了?
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